A cross sectional study of MERS CoV in camel was conducted between February 2018 to Aprilrn2019 in three selected sites of Amibara district of Afar Region, Northeast Ethiopia. The studyrnwas aimed to observe the current sero-prevalence status of MERS, assess the presence of activerncases through detection RNA viral particle using RT -PCR and investigate possible risk factorsrnfor the MERS-CoV in camels. A total of 589 sera were collected and tested with indirect Enzymernlinked Immuno Sorbent Assay (iELISA).The overall seroprevalance of MERS-CoV with this testrnwas 87.3% (n=514/589, 95% CI: 84.5-89.9). Out of the total sera samples, 198 sera werernretested by pseudo particle neutralization test (ppNT)at Hong Kong University (HKU). A total ofrn857 nasal swab samples were collected for the detection of MERS CoV RNA particle using RTPCR.rnAssociation of different risk factors with seroprevalance revealed that origin (X2=13.39,rnP=0.001), sex (X2=4.5 P=0.034), age ((X2=185.7, P=0.001) season (X2=41.7, P=0.000) andrnreproduction status (X2=96.1, P=0.001) showed a statistical significant difference for MERSrnCoV antibody detection among the groups (P0.05). In multivariable logistic regression analysis, agern(OR=7.39, 95% CI:3.43-15.91), season (OR=4.83, 95% CI:-2.14-10.90), and in adult femalerncamel reproduction status (OR=7.39, 95% CI:3.43-15.91) showed statistically significantrndifference among the groups for MERS CoV antibody detection while risk factors of origin,rnanimal sex and herd size difference were statistically insignificant. Out of the total 198 serarnsamples tested using ppNT, 197 (99.5%; 95%CI: 98.4-100) were positive where as in indirectrnELISA of 198 sera sample only 182 (91.9%; 95% CI: 88-95.7) were seropositive indicatingrnppNT is more sensitive than iELISA for MERS CoV antibody detection. Despite the presence ofrnhigh seropositivity for MERS CoV antibody, all 857 camel nasal swabs samples tested by Real-rntime reverse transcription polymerase chain reaction (RT-PCR) technique for detection MERS â€“rnCoV RNA viral particle were Negative after testing at NAHDIC and H.K.U. School PublicrnHealth laboratories .In conclusion, the present study revealed a high seroprevalance of MERSrnCoV in adult camels. However, in spite of high seroprevalance the lack of any RNA viral particlernin the present study suggests the need for further in depth longitudinal study to detect therncirculating virus focusing on juveniles and young camels whereby seroprevalance of antibody is low when compared with adult camel in order to get the active virus before the camel developrnantibody. Moreover, the zoonotic significance and potential transmission routes of MERS CoV tornpastoral communities should also be investigated and design strategy for the preparedness inrncontrol of the diseases in Ethiopia