Quarter milk samples (n = 404) from 101 traditionally managed lactating camels (Camelusrndromedarius) in Gewane district of Afar region were examined to estimate the prevalence ofrnmastitis, identification of bacterial causes associated with mastitis and determine fat and proteinrncontent of camel milk. Twenty one of the 101 (20.8%) camels examined were diagnosed asrnmastitis cases based on clinical signs. Thirty one of the 101 camels (30.7%) udders examinedrnwere infested with ticks and had lesion on the teats and udder skin, 45.2% had mastitis. Therernwere significant association (p< 0.05) between tick infestation and mastitis. Lactating camels atrnearly and mid lactation were found most affected by mastitis (p0.05). Protein percentage were significantly higher in CMTpositivernmilk samples in different parity (p