Comparison Of Diagnostic Efficacy Of Blocking Elisa With Competitive Elisa For The Detection Of Peste Des Petits Ruminants Virus Antibodies In Ruminants And Camels Sera In Ethiopia
A Peste des petits ruminant (PPR) is one of the top hindrance to small ruminantsrnproduction. To facilitate the global effort to eradicate PPR, sufficient, reliable, fast and costrneffective screening tests are important. This study compares the diagnostic performance ofrntwo anti-PPRV antibodies detection methods namely: ID Screen® PPR CompetitionrnELISA (ID Screen® PPR c-ELISA) and Haemagglutinin based PPR blocking ELISArn(HPPR b-ELISA®) kits using 480 sera collected from goats, sheep, cattle and camels. Thernresults of the two tests were validated against virus neutralization test (VNT). Thernagreements between the tests were determined using Cohen’s Kappa statistics and two-wayrncontingency table. The diagnostic sensitivity and specificity of HPPR b-ELISA® test werern79.55 and 99.74%, respectively relative to the ID Screen® PPR c-ELISA with almostrnperfect agreement (ï«=0.86) between the two tests. Conversely, the diagnostic sensitivityrnand specificity of ID Screen® PPR c-ELISA relative to HPPR b-ELISA® were 98.59 andrn95.60%, respectively. There was almost perfect agreement between the two tests in goatsrn(ï«=0.82) and sheep (ï«=0.98), while the agreement was substantial in cattle (ï«=0.78).rnHowever, there was no agreement between HPPR b-ELISA® and ID Screen® PPR cxiiirnELISA in detecting antibodies against PPRV in camels’ sera (ï«=0.00). On the other hand,rnthe results of the two tests were validated against VNT. The HPPR b-ELISA® test had arndiagnostic sensitivity and specificity of 80 and 96.36%, respectively compared to VNTrnwith substantial agreement. The agreements were almost perfect in goats (ï«=0.83) andrnsheep (ï«=0.89), moderate in cattle (ï«=0.50) and none in camels (ï«=0.00). The sensitivityrnand specificity of ID Screen® PPR c-ELISA relative to VNT were 92.00 and 76.36%,rnrespectively. The ID Screen® PPR c-ELISA test had substantial agreement in goatsrn(ï«=0.69) and sheep (ï«=0.78), fair agreement in cattle (ï«=0.30) and no agreement in camelsrn(ï«=0.00) in detecting specific antibodies directed against PPRV relative to VNT. ThernHPPR b-ELISA® test was shown to be a good screening test to be used alone or inrncombination with ID Screen® PPR c-ELISA test for PPR serological surveys orrnmonitoring in ruminants. Based on these results it can be concluded that the serology basedrnon both tests represents a reliable and valid method for detection of anti-PPRV antibodiesrnin ruminants, however, the use of ID Screen® PPR c-ELISA and HPPR b-ELISA® tests inrndetection of anti-PPRV antibodies in camels’ sera requires further investigation. Findingsrnsuggest that the newly developed HPPR b-ELISA® is suitable for screening of antibodiesrnagainst PPRV in ruminants.