A crosssectional study was conducted between November 2019 to May 2020 to estimate the seroprevalence of bovine brucellosis and associated risk factors for infection in dairy cattle in Sululta Town, Central Ethiopia. A total of 436 dairy cattle that composed of 110 males and 326 females greater than six months of age were sampled and tested for Brucella antibodies. The serum samples collected were firstly screened by using the Rose Bengal Plate test and confirmed by the Complement Fixation Test. The animal level seroprevalence was calculated as the number of seropositive samples divided by the total number of samples tested. Herd prevalence was calculated as dividing the number of seropositive herds in Complement Fixation Test with the number of herds tested. Thus the animal level seroprevalence of brucellosis in dairy cattle was 0.23% (95% CI: -0.2-0.7%) and herd-level was found to be 0.8% (95% CI: -0.08- 0.2%) by Complement Fixation Test. A Chi-square computed statistical analysis indicated that large herd size (> 20 animals) have a significantly association with the seroprevalence of bovine brucellosis as compared to small herd size and medium herd size (χ2=17.20; P