Molecular Surveillance Of Equine Herpes Virus -1 2 And 5 Circulating In Working Equids In Central Ethiopia

Equine herpesvirus infections have a major economic, health, and welfare impact onrnworking equids worldwide.This study was performed from October 2019 to April 2020 inrnthe North Showa of Amhara regional state, East Shewa and West Arsiof Oromia regionalrnstate of Ethiopia,for molecular detection of the virus,and to identify the associationrnbetween expected risk factors and occurrence of EHV -1,2 and 5 infectionsamongrnclinically suspected working equine population. A total of 58 samples were collected fromrn33 donkeys and 25 horses suspected with clinical signs of EHVs infection.Detection ofrnEHV-1, 2, and 5 genes in the collected samples was done using polymerase chain reactionrn(PCR).Assessment of the associated risk factors was conducted using a multivariablernlogistic regression model.The results of this study showed that out of the 58samples, 36rn(62%), 31(53%), and 15(25%) were found positive for EHV-1, 2, and 5, respectively.rnConcurrent infections with EHV-1 and EHV-2 (n = 18; 31%), EHV-1 and EHV-5 (n = 10;rn17%), EHV-2 and EHV-5 (n= 9; 15.5%), and EHV-1, 2, and 5 (n = 8; 13%)werernrecorded.There wasno statistically significant difference (P > 0.05) of EHV-1, 2, and 5rninfections with regard to age, sex, body condition score, and study sites, butstatisticallyrnsignificant difference (P

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