The aim of this study was to quantify the prevalence, determine associated risk factors andrnisolate Staphylococci species and determine its resistance to various antimicrobial agents inrncattle, food, equipments and humans in central Ethiopia. In order to isolate and identifyrnStaphylococci species from abattoir and dairy cows a cross- sectional study was conductedrnfrom October 2013 to May 2014. The results showed that the prevalence of Staphylococcirnspecies were 47.1% (140/297) udder milk, 58% (29/50) tank milk of the selected dairy farms,rn34.4% (17/50) cow bucket swab, 38% (19/50) farm tank swab, 38% (19/50) hand and 70%rn(12/17) nasal swab of milkers, 38.5%(139/361) carcass swab, 37.8% (14/37) knife swab,rn48.6% (18/37) slaughter line swab, 37.8% (14/37) hand and 46.7% (7/15) nasal swab ofrnbutchers with over all prevalence of 42.8%(428/1001). In addition, the prevalence ofrnStaphylococci were 48.6% (88/181), 47.7% (92/193), 40.7% (103/253), 40.2% (78/194) andrn37.2% (67/180) in Assela, Addis Ababa, Bishoftu, Holeta and Adama respectively. The S.rnaureus was the most frequently isolated species among different samples accounting for 172rn(17.2%) followed by S. hyicus 82 (8.2%); S. intermedius 74 (7.4%) and CNS 96 (9.6%). Riskrnfactors analysis revealed that prevalence of Staphylococcus significantly differed with sourcernof sample (P < 0.05) and type of sample (P < 0.05) in central Ethiopia. Prevalence ofrnStaphylococcus were significantly higher (p