Detection and monitoring of insecticide resistance in malaria vectors is crucial and has to be conducted together with other entomological indices for effective vector control. The aim of this study was to identify species composition of Anopheles mosquitoes, behaviour and feasibility of resistance management using rotation of indoor insecticides spray combined with different insecticide impregnated long lasting insecticidal nets. Monthly larvae and adult Anopheles collections were undertaken from May 2013 to June 2015 in Intervention (Jolie) and Control (Gogete) study sites. Identification of Anopheles species were done using morphological keys under a compound microscope as well as 10% of the adult specimens identified as Anopheles gambiae s.l. also subjected for species-specific polymerase chain reaction (PCR) test for sibling species detection. Abdominal status, host and resting preferences, indoor resting density, parity rate, sporozoite rate, man biting rate and entomological inoculation rate were compared among the Intervention (Jolie) and Control (Gogete) study sites to evaluate the impact of the resistance management option used in Intervention study site (Jolie). Besides, An. gambiae s.l. susceptibility tests result carried out every year throughout the study period (in the month of August 2013, 2014, 2015 and 2016) to determine the susceptibility status of An. gambiae s.l. among the Intervention (Jolie) and Control (Gogete) study sites were compared to observe the impact of the resistance management option. To detect the presence of the L1014S and the L1014F kdr mutation a sub-sample of An. arabiensis and An. gambiae were selected from both study sites and tested using adapted versions of the allele-specific polymerase chain reaction (from October 2016 to April 2017). xx A total of 4118 third and fourth instars larvae of Anopheles mosquitoes used for identification, ten in Intervention study site (Jolie) and eleven in Control (Gogete) Anopheles species were identified. Most of Anopheles larvae (27.5%) were collected from marshy breeding site which served as major breeding sites during the study period, followed by irrigation canals (24.5%), rain pools (20.4%), river pockets (17.6%) and ponds (9.9%). In both Intervention (Jolie) and Control (Gogete) study sites the predominant Anopheles species was An. gambiae s.l. which is the main vector in the country. Statistically significant (p