Human-induced stressors (urbanization, agriculture, and industrial effluent) are common around Lake Ziway. As a result, the lake is threatened and ecological functions of the lake are in question, and this requires urgent monitoring. Therefore, this study was conducted to assess the ecological quality of Lake Ziway using a composite diatom and macroinvertebrate multimetric index. Physicochemical and biological data were collected from 9 sites in the littoral zone between September 2015 and April 2016 in 4 rounds. Candidate references were proposed through a priori selection criteria. Physicochemical parameters were different among sites, except for pH, soluble reactive phosphorus (SRP), and NH4+. pH showed no significant change among sites as well during the last two decades. DO, EC, Total Phosphorus (TP), and NO2, showed variation among the sites as the mean values ranged from 3.51±0.27 to 9.33±0.30 mg/L, 405±0.5 to 592±75.6 μS/cm, 0.14±0.02 to 0.51±0.45 mg/L, and 0.03±0.02 to 0.22±0.21 mg/L, respectively (p