The exotic stemborer, Chilo partellus (Swinhoe) (Lepidoptera: Crambidae) is the most important pest of sorghum in East Africa. The insect infests sorghum crop throughout its growth stages. It has also been recorded and documented from wild host plants. C. partellus has several native and exotic natural enemies. Field and laboratory studies were conducted to know the status of C. partellus and its natural enemies and assess the efficacy of botanicals and cow urine against C. partellus and their effect on the natural enemies. Surveys were conducted in 2016/17 and 2017/18 in three districts and nine farmer fields of Kalu, Bati and Dawa Chefa districts, Ethiopia. Sampling was done both randomly and purposively. Analysis of variance (ANOVA) was carried out to determine if there were any significant differences across the locations and between the plant growth stages. Laboratory evalution of plant and animal-based insecticides and optimum concentration on C. partellus was conducted in 2016. The experiment was laid out in completely randomized design in three replications for each rate. The rates were 2g, 2.5g, 3g of powder and 1ml, 1.5ml and 2ml w/v of the solutions. The most effective biopesticides were further tested in 2017 in farmerâ€™s field. The trial was designed in a randomized complete block design in factorial arangment in three replications. The treatments were at two levels of frequencies (2 & 3 times application) and three rates of powder (1g, 2g and 3g) and three rates of solution (5%, 10% and 15%). The study revealed that C. partellus constituted 90.3 & 91.2% on sorghum and 45.4 & 69.2% on wild hosts in 2016/17 and 2017/18, respectively. Significant differences were observed among the host plants and sorghum growth stages. In sorghum, C. partellus density were found to be significantly higher than on wild hosts (P