Evaluation Of Trichoderma Species Against Fusarium Wilt And Alternaria Leaf Blight Of Sesame (sesamum Indicum L.) Under In Vitro Condition

The present study was aimed to evaluate and characterize the potential biocontrol agents of Trichoderma isolates against two pathogenic fungi viz., Alternaria isolates (AUA1) and Fusarium isolates (AUF5) under in vitro condition. Infected leaves, seeds and soil samples of sesame were collected from different habitats of the Wolkait district for the isolation of fungal pathogens. The pathogenicity test of these isolates was confirmed on detached leaves of sesame plants resulting in typical symptoms of leaf blight and wilt disease. In this study, the in vitro potential of 7 Trichoderma isolates were evaluated against two isolates of foliar and soil borne phytopathogenic fungi in dual culture techniques and through production of volatile and non-volatile inhibitors. The effect of pH and temperature on the mycelia growth and spore yield of Trichoderma isolates were determined PDB medium. While, the optimum temperature for mycelial growth and the maximum spore yield was produced at 25OC. Among the seven Trichoderma isolates, AUT-12 (93 %), AUT-158 (91.6 %) and AUT-97 (90.8%), exhibited a significant (P< 0.05) enhancement of germination percentage in sesame seeds under in vitro conditions, while the control treated significantly (p

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