Gullele Botanic Garden contains diverse types of invertebrates. The diversity,rndistribution, frequency, density and abundance of these invertebrates were investigatedrnusing 28 quadrats which were located at different habitat types. The study found thatrnGullele Botanic Garden support the presence of invertebrates in large quantity in mixedrnforest habitat. A total of 2854 invertebrate specimens belonging to 42 families were collectedrnand recorded in the Gullele Botanical Garden. Higher diversity and evenness ofrninvertebrates were observed in the mixed foresthabitat. On the other hand, the least evennessrnand diversity of invertebrates was observed in the grassland forest habitat in the studyrnseason. Based on jaccard's similarity index, the highest similarity was observed betweenrnmixed forest and grassland forest habitats.. The lowest similarity was observed betweenrnnatural forest habitat and artificial forest habitat. Abundance of food on species ofrnvegetation, stability of the habitats, human activities and settlement inside determinedrninvertebrate diversity in the study areas.During data collection in Gullele Botanic Garden,rncareful observation showed that invertebrates had both positive and negative effect forrnhumans, animals and insects. The positive effects were increasing soilrnfertility(earthworms),nutrient cycling(ants),increasing the rate of soil nutrientrncycling(beetles) and indicators of environmental change and habitat disturbance(insects).rnConversely, the negative effects were plant drying(termites) and attackingrnroots,trunks,stems,leaves, flowers ,seeds ,fruits and saps of vascular system(phytophagousrninvertebrates).