A comparative study was carried out the disturbances by humans on thernEthiopian wolf (Canis simensis) in Chilalo-Galama Range and Sanetti Plateaurnduring July, 2009- March, 2010. Factors disturbing the Ethiopian wolf wererninvestigated using a questionnaire survey and by direct observations.rnDescriptive statistics, Spearman correlation coefficient, one-way ANOVA, Tukeyrnand Chi-square tests were used to analyze the mean amount of factorsrndisturbed the Ethiopian wolf and compared the differences existing among thern15 transects and four wolf packs. The analysis indicated that 62.7% and 100%rnof the Chilalo-Galama and Sanetti Plateau respondents, respectively hadrnpositive attitude towards the wolf. In the Chilalo-Galama, 488(99%) and in thernSanetti Plateau, 5(1%) of people were observed disturbing the wolves whilerncollecting fuel, grass and herding livestock. On the other hand, 229.8 Â± 149.67rnand 92.93 Â± 49.3 mean of livestock per transect foraged in the study areasrnduring the wet and dry seasons, respectively. There was a significancerndifference in the amount of livestock grazed in the wolf habitats per transect (Frn3 11 = 13.56, P < 0.001), wet season and (F 3 11 = 3.96, P < 0.039), dry season.rnThere was a serious wolf disturbance by humans (Ï‡2 = 34, df. 1, P < 0.05) inrnmaking noise and (Ï‡2 = 4, df. 1, P < 0.05) in chasing after the wolf in thernChilalo-Galama than in the Sanetti Plateau. Therefore, due attention should berngiven to alleviate the problem.rnKey words/phrase: Chilalo-Galama; Ethiopian wolf; human interaction;rnSanetti Plateau.