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Science Lab Technology Project Topics and Research Materials

THE ANTIBACTERIAL ACTIVITY OF THREE TYPES OF MEDICATED SOAPS ON STARHYROCOCCUS AUREUS FORM WOULD INFECTIONS

ABSTRACT

 

The antibacterial activity of three type of medicate soap on staphylococcus aureus isolated from wound infections was conducted. Fifty individual with would infection within the age range of 9-73 years were sampled. Swabsticks were used to collect specimens from wound infection. each swabstick was streaked separately over plates of nutrient   and  macconkey agar and later incubated at 370c. out of the fifty (50) individuals sampled 25(50%) were mostly infected with staphylococcus aureus followed by pseudomonas acruginqu (22%)  staphylococcus epidermis (10%) least by Exchericha coli b(12%) The organisms occur within the age range 9-13 years with 8 (16%) followed by 14-18 years 4(8%) next by the age range of 19-23 years 3(6%) followed by 24-28 years with 2(4%) followed 24-28 years with 2(4%) and the heart in the age range are 29-33,34-38,39-43,44-48,49-53,54-58 64-68 with 1(2%) each while there was no isolation in the age range 59-63. The study also revealed that all the staphylococcus aureus isolated were sensitive to the three medicated soap. The means of inhibition was highest in Aleppo medicated soap with 12.92 mm followed by temperate medicated soap with 11.2mm and least in carat medicated soap with 10.56 mm. Also the staphylococcus aureus isolated were also sensitive to the commercial autibutics used as couplers the means zones of inhibition was highest in ampiclox with 12.8 mm followed by gentamyain with 12.04 mm and the heart in penicillin with 10.4 mm. there was a close relationship in the zenes of inhibition between the three medicated soap and the commercial antibiotics used as coconutrels. The result showned that medicated soaps were effective against staphylococcus aureus involved in would infection in humans and  are comparable to commercial antibiotics. There fore medicated soap can be used in cleaning the skin particularly in the affected part of the skin during the time one has hurt or cut on the  skin.

 

 

 

 

 

 

 

 

TABLE OF CONTENTS

 

Title page

Certification

Dedication

Acknowledgement

Abstract

Table of contents

List of table

 

CHAPTER ONE

1.0            Introduction

1.1            Aims and objectives

1.2            Statement of the problem

1.3            Hypothesis

1.4            Justification of the study

1.5         Limitation Of The Study

 

 

CHAPTER TWO

2.0            Literature review

2.1            Antibacterial activity of soap

2.2            Medical relevance of antimicrobial soap

2.3            Normal skin microbiota and soap

 

CHAPTER THREE

3.0            Material and methods

3.1            Materials

3.2             sterilization

3.2.2    Collection of soap sample

3.2.3    Preparation of culture media

3.2.4    Collection of bacterial organisms from would infection

3.2.5    Microbial count, grams stain and microscopic examination

3.2.6    Biochemical test for identification of isolates

 

 

 

CHAPTER FOUR

Result

 

CHAPTER FIVE

Discussion

 

CHAPTER SIX

Conclusion and recommendation

References

Appendix

 

 

 

 

 

 

 

 

 

 

LIST OF TABLE

 

Age distribution number sampled as well as percentages with staphylococcus aureus and other organisms

Table 2

Age distribution as well as percentage of each isolated organisms

Table 3

Preliminary identification of bacterial isolates

Table 4

Biochemical test carried out for identification of bacterial isolates

Table 5

Sensitivity test using three medicated soap and three antibiotics indicating their zones of inhibition.

 

CHAPTER ONE

 

INTRODUCTION

According to pelage et al (1986) antibacterial activity is the ability to either destroy bacteria or inhibit their growth. This is significant with respects to the human body in preventing sepsis and skin infections. Also Derland (1981) states that medicated soap have the germicidal substance like chloroxyhlenol potassium mercuric iodide, trichlorocarbanlide etc. incorporated into them, in order to enhance their antibacterial activity. These germicide  substance are normally added in a specified amount and percentage of the substance used are always stated on the soapcase or  inside the lesflet which certains the information on how to use the soap for various purposes.

Anon (1964) states  that soap may be defined as a chemical compound resulting from the interaction of fathy acids oil and caustic soda (alkali) possesing the characteristic soap like properties of detergents, surface tension lowering wetting and emulsifying power and gel formation. All fatty oils and fats. Are mixtures of glycoside compounds (erters) of trihydric alcohol, glycosides and some  fatty acids such as plasmatic acid the chemistry of soap manufacturing may  be expressed the following equation which is know as saponification (Anon (1964).

HzCocoG5H31)3 + Na 0H          Naoco15H31(zH31 + c3H5(oH)3

 

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