This work was done in order to investigate the microbial content of some bolted soft drivels sold in Enugu interlopes. Soft drink are consumed in large quantities by the different age groups, adult and children of different class of people all over the state. This is aided by the general availability of these drink almost every place roleve people gather for work or recreational activities. It is the appropriate to subject these soft drinks to some kind of investigation by isolation and characterizing the microorganism found.

Simple Grows staining and biochemical test method were employed to isolate and identify the organisms found different bacterial and feast were identified these organisms include Fvlavobacterium spp. Bacillus Eschericjhism coli, Enteribacter arccosines Micro coccusroseus Micrococcus various Bacillus subtitles Aciuetobacter iwoffi, Micrococcus inteus Bacillus polymea, flavobacterium, rigeuse sacchenomyces, conevisciae klurenomyce sff, Torulopsis spp, cadida valida Geotrichuin candidium, sacchanoyces, carlbergersis cryptococus, albidus, Hausennlsa spp etc.

TABLE OF CONTENT

Title page

Certification

Dedication

Acknowledgement

Abstract

Table of content

List of table

1.0 CHAPTER ONE

1.1 Introduction

2.0 CHAPTER TWO

2.1 Literature review

2.1.1 History and overview of soft drink industries

2.1.2 Production of soft drinks

2.2 Microbiology of soft drinks

2.3 Microbial growth

2.4 Factors affecting microbial growth

2.5 Ingredients and their effects

CHAPTER THREE

2.6 Material and method

2.7 Sample collection

2.8 Sterilization of glass warier.

2.9 Preparation of dividend and media

3.4.2 Preparation of serial / isolation

2.10 Identification and characterization of isolate organism

2.10.1 Colonial morphology

2.10.2 Cellular morphology

2.10.3 Grannies straining

2.11 Biochemical characteristic

2.11.1 Oxidase test

2.11.2 Citrate utilization test

2.11.3 Starch hydrolysis test

2.11.4 Nitrate reduction

2.11.5 Starch fermentation

3.0 CHAPTER FOUR

4.1 Result and discussion

4.0 CHAPTER FIVE

4.1 Conclusion and recommendation

Appendix

References

LIST OF TABLE

Table 1: colony counting

Table 2: cultural characteristic of bacterial growth on nutrient agar

Table 3: cultural characteristic of yeast growth on Pdp agar.

Table 4: result obtaining from gram staining and cellular morphology of bacterial isolates.

Table 5: result obtained from gram staining and cellular morphology of yeast isolates.

Table 6: different bacterial identified from the result of biochemical test (Appendix A& B)

Table 7: organisms (yeast) identified from the result of biochemical test. (appendix C & D).

CHAPTER ONE

1.1 INTRODUCTION

Soft drinks were defined by Harry and Wilbert (1984) as a class of non alcoholic beverages which may be divided into two classes carbondrate soft drinks some time referred to as Soda ;’soda pop “etc) and still or non- carbondrate soft drinks. soft drinks in general contains 86 to 92% water, 7 to 10% nutrient sweetbriers, carbodioxide (if present) acid and flavoring. The food and drug administration standard of identity for carbonated soft drink define them as a class of beverage made by absorbing carbodiocide in potable water. The amount of carbonhdioxide used is not less than that which well be absorbed by the beverage at a pressure of one atmosphere and a temperature of 60F. these drinks contain no alcohol, or only such alcohol (not in excess of 0.5% by weight of the finished beverage) as is contributed by the flavoring ingredient that may be use in carbonated soft drinks in such properties are reasonable required to accomplish their intruded effects. These include nutritive sweeteners flavoring ingredient natural and antificial colour additive acidifying agent buffering agent emulsifying stabilizing or viscosity producing agent foaming agent caffeine and chemical preservatives the beginning of the carbonated beverage (soft) drinks) as recorded by Resrosier (1977) date back to the closing years of the eighteen (18^th ) century

Morris (1959) defined micro organisms as plant or animal organisms of microscopic or submicroscopic dimensions. Plant microorganisms are generally classified into three major groups, namely fungi bacteria and Algae as for as beverage manfacture is concerned the fungi are the most important group of spoilage organisms because yeast and mould belong to this group. The protozoa are the most important of the animal microorganisms.

Actually individual microorganisms are microscopic in size they can form colonies clouds precipitates sediments and scums with time and these are visible to the naked eyes and are the direct signs of the presence of microorganisms.

The microbiological safety and ratability of the diverse type of soft drinks products depend on their formulation (including) the use of chemical preservative carbonation low PH values and pasteurization) this was stated by ganglion (1982). Batchelor (1985) put it that the inter related environmental factors that determine the microbial load of product or the susceptibility of a product to microbial spoilage are intrinsic and Extrinsic factors.

Intrinsic factor are derived from the product and its formulation according to pauezai comments they are not easy to change Extrinsic factor are variable